Quantifying uncertainty in spikes estimated from calcium imaging data
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In recent years, a number of methods have been proposed to estimate the times at which neurons spike on the basis of calcium imaging data. However, quantifying the uncertainty associated with these estimated spikes remains an open problem. We consider a simple and well-studied model for calcium imaging data, which states that calcium decays exponentially in the absence of a spike, and instantaneously increases when a spike occurs. We wish to test the null hypothesis that the neuron did not spike – i.e., that there was no increase in calcium – at a particular timepoint at which a spike was estimated. In this setting, classical hypothesis tests lead to inflated Type I error, because the spike was estimated on the same data. To address this problem, we propose a selective inference approach to test the null hypothesis. We describe an efficient algorithm to compute finite-sample p-values that control selective Type I error, and confidence intervals with correct selective coverage, for spikes estimated using a recent proposal from the literature. We apply our proposal in simulation and on calcium imaging data from the spikefinder challenge.
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