Differentiable model-based adaptive optics for two-photon microscopy

04/29/2021
by   Ivan Vishniakou, et al.
0

Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. Such models can be combined with the optimization techniques of machine learning frameworks to find aberration corrections, as was demonstrated for focusing a laser beam through aberrations onto a camera [arXiv:2007.13400]. Here, we extend this approach to two-photon scanning microscopy. The developed sensorless technique finds corrections for aberrations in scattering samples and will be useful for a range of imaging application, for example in brain tissue.

READ FULL TEXT

page 5

page 6

page 7

research
07/27/2020

Differentiable model-based adaptive optics with transmitted and reflected light

Aberrations limit optical systems in many situations, for example when i...
research
04/09/2020

Adaptive optics with reflected light and deep neural networks

Light scattering and aberrations limit optical microscopy in biological ...
research
11/30/2022

Differentiable optimization of the Debye-Wolf integral for light shaping and adaptive optics in two-photon microscopy

Control of light through a microscope objective with a high numerical ap...
research
02/13/2018

Sources of Variance in Two-Photon Microscopy Neuroimaging

Two-photon laser scanning microscopy is widely used in a quickly growing...
research
07/08/2020

Guidestar-free image-guided wavefront-shaping

Optical imaging through scattering media is a fundamental challenge in m...
research
04/26/2023

Automated Whole Slide Imaging for Label-Free Histology using Photon Absorption Remote Sensing Microscopy

The field of histology relies heavily on antiquated tissue processing an...

Please sign up or login with your details

Forgot password? Click here to reset